A simple, accurate and precise spectrophotometric method was developed for simultaneous determination of emtricitabine and tenofovir alfenamide in bulk and their combined pharmaceutical dosage form. Emtricitabine shows maximum absorbance at 280 nm and tenofovir alfenamide shows maximum absorbance at 260 nm. Regression analysis of Beer’s plots showed good linearity in concentration range of 2.5 μg/ml for emtricitabine and 5-30 μg/ml for tenofovir alfenamide with correlation coefficient 0.999 and 0.998 for EMT and TAF respectively. The mean percent label claim of tablets of emtricitabine and tenofovir alfenamide in formulation estimated by the proposed method was found to be 99.75% and 100.8% for EMT and TAF respectively. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical parameters were found to be good accordance with the prescribe values. The amount of drug recovered was calculated and the percentage recovery was found to be in the range of 99-101% for EMT, 98 -101 % for TAF. The precision (intra-day, inter-day and repeatability) of method was found within limits for both the drugs. The proposed method was successfully applied for analysis of these drugs in commercial tablets.
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